Effect of lifelong football training on the expression of muscle molecular markers involved in healthy longevity
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Effect of lifelong football training on the expression of muscle molecular markers involved in healthy longevity. / Mancini, A; Vitucci, D; Labruna, G; Imperlini, E; Randers, Morten Bredsgaard; Schmidt, Jakob Friis; Hagman, H. ; Andersen, Thomas Rostgaard; Russo, R; Orrù, S; Krustrup, Peter; Salvatore, F; Buono, P.
I: European Journal of Applied Physiology, Bind 117, Nr. 4, 2017, s. 721-730.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Effect of lifelong football training on the expression of muscle molecular markers involved in healthy longevity
AU - Mancini, A
AU - Vitucci, D
AU - Labruna, G
AU - Imperlini, E
AU - Randers, Morten Bredsgaard
AU - Schmidt, Jakob Friis
AU - Hagman, H.
AU - Andersen, Thomas Rostgaard
AU - Russo, R
AU - Orrù, S
AU - Krustrup, Peter
AU - Salvatore, F
AU - Buono, P
N1 - CURIS 2017 NEXS 076
PY - 2017
Y1 - 2017
N2 - PURPOSE: We investigated whether lifelong football training affects the expression of healthy longevity-related muscle molecular markers.METHODS: Biopsies were collected from the vastus lateralis muscle of 10 lifelong football-trained men (68.2 ± 3.0 years) and of 10 active untrained healthy men (66.7 ± 1.3 years). Gene and protein expression was measured by RTqPCR on RNA and by western blotting on protein extracts from muscle biopsies, respectively.RESULTS: The expression of AMPKα1/α2, NAMPT, TFAM and PGC1α, which are markers of oxidative metabolism, and MyHC β isoform expression was higher in the muscle of football-trained men vs untrained men. Also citrate synthase activity was higher in trained than in untrained men (109.3 ± 9.2 vs 75.1 ± 9.2 mU/mg). These findings were associated with a healthier body composition in trained than in untrained men [body weight: 78.2 ± 6.5 vs 91.2 ± 11.2 kg; body mass index BMI: 24.4 ± 1.6 vs 28.8 ± 4.0 kg m(-2); fat%: 22.6 ± 8.0 vs 31.4 ± 5.0%)] and with a higher maximal oxygen uptake (VO2max: 34.7 ± 3.8 vs 27.3 ± 4.0 ml/min/kg). Also the expression of proteins involved in DNA repair and in senescence suppression (Erk1/2, Akt and FoxM1) was higher in trained than in untrained men. At BMI- and age-adjusted multiple linear regression analysis, fat percentage was independently associated with Akt protein expression, and VO2max was independently associated with TFAM mRNA and with Erk1/2 protein expression.CONCLUSIONS: Lifelong football training increases the expression of key markers involved in muscle oxidative metabolism, and in the DNA repair and senescence suppression pathways, thus providing the molecular basis for healthy longevity.
AB - PURPOSE: We investigated whether lifelong football training affects the expression of healthy longevity-related muscle molecular markers.METHODS: Biopsies were collected from the vastus lateralis muscle of 10 lifelong football-trained men (68.2 ± 3.0 years) and of 10 active untrained healthy men (66.7 ± 1.3 years). Gene and protein expression was measured by RTqPCR on RNA and by western blotting on protein extracts from muscle biopsies, respectively.RESULTS: The expression of AMPKα1/α2, NAMPT, TFAM and PGC1α, which are markers of oxidative metabolism, and MyHC β isoform expression was higher in the muscle of football-trained men vs untrained men. Also citrate synthase activity was higher in trained than in untrained men (109.3 ± 9.2 vs 75.1 ± 9.2 mU/mg). These findings were associated with a healthier body composition in trained than in untrained men [body weight: 78.2 ± 6.5 vs 91.2 ± 11.2 kg; body mass index BMI: 24.4 ± 1.6 vs 28.8 ± 4.0 kg m(-2); fat%: 22.6 ± 8.0 vs 31.4 ± 5.0%)] and with a higher maximal oxygen uptake (VO2max: 34.7 ± 3.8 vs 27.3 ± 4.0 ml/min/kg). Also the expression of proteins involved in DNA repair and in senescence suppression (Erk1/2, Akt and FoxM1) was higher in trained than in untrained men. At BMI- and age-adjusted multiple linear regression analysis, fat percentage was independently associated with Akt protein expression, and VO2max was independently associated with TFAM mRNA and with Erk1/2 protein expression.CONCLUSIONS: Lifelong football training increases the expression of key markers involved in muscle oxidative metabolism, and in the DNA repair and senescence suppression pathways, thus providing the molecular basis for healthy longevity.
KW - Veteran football players
KW - Long-term football training
KW - Healthy aging
KW - DNA repair and senescence suppression
U2 - 10.1007/s00421-017-3562-8
DO - 10.1007/s00421-017-3562-8
M3 - Journal article
C2 - 28251397
VL - 117
SP - 721
EP - 730
JO - European Journal of Applied Physiology
JF - European Journal of Applied Physiology
SN - 1439-6319
IS - 4
ER -
ID: 173948441